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Wafaa Moustafa El Kady

Basic information

Name : Wafaa Moustafa El Kady
Title: An Associate professor of Pharmacognosy
Google Schoolar Link
Personal Info: Dr. Wafaa Elkady, An Associate professor of Pharmacognosy, Pharmacognosy and Medicinal Plants Department, She got her Ph.D. at Helwan University

Education

Certificate Major University Year
PhD Department of Pharmacognosy and Medicinal Plants Faculty of Pharmaceuitical Scince -Helwan University 2015
Masters Pharmacognosy Department Helwan University - Faculty of Pharmacy 2009
Bachelor . Helwan Univesity - Faculty of Pharmacy 2002

Teaching Experience

Name of Organization Position From Date To Date
Modern Science and Arts University Teaching Assistant in Microbiology and Pharmacognosy Department 01/08/2005 01/01/2007

Researches /Publications

Impact of Altitudinal Variation on the Phytochemical Profile, Anthelmintic and Antimicrobial Activity of Two Pinus Species - 01/0

Wafaa Mostafa Hussein Ali Elkady

Mariam H. Gonaid , Miriam F. Yousif , Mahmoud El-Sayed and Hind A.N.Omar

01/05/2021

Active components from natural sources are the current focus in most pharmacological research to provide new therapeutic agents for clinical use. Essential oils from the Pinus species have been traditionally used in medicine. This study aimed to investigate the chemical profile of two Pinus species, Pinus halepensis L. and Pinus pinea Mill, from different altitudes in Libya and study the effect of environmental conditions on the biological activities of essential oils. A clevenger apparatus was used to prepare the essential oils by hydrodistillation. Analyses were done using GC/MS. Anthelmintic and antimicrobial activities were tested against the earthworm Allolobophora caliginosa, gram-positive bacteria, gram-negative bacteria, and fungi. Different chemical profiles were observed among all tested essential oils, and terpenes were the most dominant class. All studied essential oils from the Pinus species exhibited a remarkable anthelmintic activity compared to the standard piperazine citrate drug. Pinus halepensis from both altitudes showed broad-spectrum antimicrobial activity against all tested microorganisms, while Pinus pinea was effective against only Escherichia coli. From these findings, one can conclude that there are variations between studied species. The essential oil compositions are affected by environmental factors, which consequently affect the anthelmintic and antimicrobial activity

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Identification and isolation of anti-pneumonia bioactive compounds from Opuntia ficus-indica fruit waste peels. - 01/0

Wafaa Mostafa Hussein Ali Elkady

Bishr MM, Abdel-Aziz MM

01/05/2020

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Euryops pectinatus L. Flower Extract Inhibits P-glycoprotein and Reverses Multi-Drug Resistance in Cancer Cells: A Mechanistic Study - 01/0

Wafaa Mostafa Hussein Ali Elkady

Iriny M. Ayoub, Yousra Abdel-Mottaleb, Mohamed F. ElShafie and Michael Wink

01/02/2020

Euryops pectinatus is a South African ornamental plant belonging to family Asteraceae. The present work evaluates the cytotoxic activity and phytochemical profile of the flower extract. Metabolite profiling was performed using HPLC-PDA-ESI-MS/MS. Total phenolics and flavonoids content were assessed. Cytotoxicity was evaluated against 6 different cancer cell lines using MTT assay. The possible underlying mechanism was proposed. We analyzed whether the extract could overcome the resistance of multidrug-resistant cancer cells for doxorubicin. The effect of combination of E. pectinatus with doxorubicin was also studied. Additionally, the potential inhibitory activity of the identified phytochemicals to PB1 protein was analyzed using in silico molecular docking. Twenty-five compounds were tentatively identified. Total phenolic and flavonoid contents represented 49.41 ± 0.66 and 23.37 ± 0.23 µg/mg dried flower extract, respectively. The extract showed selective cytotoxicity against Caco2 cells but its main effect goes beyond mere cytotoxicity. It showed strong inhibition of P-glycoprotein, which helps to overcome multidrug resistance to classical chemotherapeutic agents. In silico molecular docking showed that dicaffeoyl quinic acid, kaempferol-O-rutinoside, rutin, and isorhamnetin-O-rutinoside exhibited the most potent inhibitory activity to PB1 involved in tumor progression. Euryops pectinatus flower heads could have promising selective cytotoxicity alone or in combination with other chemotherapeutic agents to counteract multidrug resistance.

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Phytochemical profile and protective effect of Ocimum basilicum aqueous extract in doxorubicin/irradiation‐induced testicular injury. - 01/0

Wafaa Mostafa Hussein Ali Elkady

Ibrahim RY

01/01/2020

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Phytochemical profile and protective effect of Ocimum basilicum aqueous extract in doxorubicin/irradiation‐induced testicular injury - 01/1

Wafaa Mostafa Hussein Ali Elkady

Rasha Y. M. Ibrahim

01/10/2019

Objectives Combined chemotherapy and radiotherapy usually associated with various comorbidities especially on rapidly proliferating cells as testis. This study aimed to characterize main constituents of Ocimum basilicum L. (OB) aqueous extract and examine its protective effect on doxorubicin/irradiation (DOXO/IR)‐induced testicular injury in rats. Methods Spectrophotometric analysis showed considerable amount of polyphenolic (146.31 µg/mg) and flavonoid contents (28.63 µg/mg); UPLC‐ESI‐MS/MS analysis revealed that the major flavonoid was apigenin‐O‐glucoside (7.53%) followed by luteolin (5.94%), while rosmarinic acid was the major polyphenolic (15.76%) followed by caftaric acid (9.39%); rutin and querctin were also present and were quantified using high‐performance liquid chromatography. Administration of OB extract (200 mg/kg per day; p.o.) to DOXO/IR rats resulted in marked improvement of associated testicular damage. Key findings Ocimum basilicum L. significantly decreased testicular levels of nuclear factor‐kappa B and B‐cell lymphoma‐2 (Bcl2)‐associated protein X, along with caspase‐3 immunohistochemical staining. In addition, OB elevated testicular total antioxidant capacity, nuclear erythroid‐related factor‐2, Bcl2 and testosterone contents and Ki‐67 immunohistochemical staining. Such changes were also accompanied by restoration of testicular architecture. Conclusions The study highlights the protective role of OB aqueous extract in hampering most of the harmful chemotherapy/radiotherapy‐induced outcomes via its antioxidant, antiapoptotic and cell regeneration abilities. Such findings may offer an incentive in expanding its use during chemotherapy and radiotherapy.

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Difference in chemical composition and antimicrobial activity of Thymus capitatus L. essential oil at different altitudes - 01/1

Wafaa Mostafa Hussein Ali Elkady

Lamya F.A. El-Jalel , Mariam H. Gonaid , Kamel A. El-Gareeb

01/12/2018

The aim of the present study is to investigate the effect of different altitude on the chemical composition and antimicrobial activity of the essential oil prepared from wild Libyan growing Thymus capitatus L. aerial parts collected from Al-Jabal Akhdar. Two different altitudes were chosen represented by Abu-Draa (650 m above the see level) and Sidi Al-Hamey (850 m above the see level). This study concerned with both qualitative and quantitative variations. The percentage yield for T. capitatus essential oils yield were 1.5% and 1.04% for both Abu-Draa and Sidi Al-Hamry respectively. GC/MS analysis for each essential oil revealed the identification of 14 volatile components corresponding to 91.99% from Abu-Draa and 23 compounds corresponding to 96.54% from Sidi Al-Hamrey. Carvcrol was the major volatile component present in both essential oils of Abu-Draa and Sidi Al-Hamrey regions (58.56% and 24.28% respectively). The second major volatile compound present in the essential oil prepared from Abu-Draa is b-caryophyllene (7.41%) followed by its oxide (6.26%); on the other hand Gamma-terpinene is the second major volatile compound present in Sidi Alhamry prepared oil (16.18%) followed by caryophyllene oxide (10.43%). Both phenolic and phenolic ethers are dominated in both examined oils (62.51, 33.74% respectively) resulting in a great antimicrobial activity for both of them which is more prominent in that oil prepared from the low altitude Abu-draa.

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Chemical profiling and antiproliferative effect of essential oils of two Araucaria species cultivated in Egypt - 01/0

Wafaa Mostafa Hussein Ali Elkady

Iriny M. Ayoub

01/03/2018

Araucaria is a small genus of evergreen coniferous trees, widely used for ornamental and timber purposes. Araucaria species are rich in essential oils. In this study, the essential oils obtained from the foliage of Araucaria heterophylla (Salisb.) and Araucaria bidwillii Hook were analyzed using GC and GC/MS. Cytotoxicity of both essential oils was assessed on three human cancer cell lines using MTT assay. Twenty nine compounds were annotated in A. heterophylla essential oil, whereas forty three were identified in A. bidwillii accounting for 95.16% and 81.06% of the total volatile oil composition, respectively. α-Pinene (70.85%), D-limonene (4.26%) and germacrene D (2.99%) represented the major compounds in A. heterophylla. Beyerene (35.65%), transnerolidol (13.66%) and ɣ-elemene (6.09%) dominated in A. bidwillii. Both oils showed potent cytotoxicity against all tested cancer cell lines (Hep-G2, MCF-7 and Caco-2) exhibiting IC50 values of 0.70, 3.20 and 1.10 μg/ ml for A. heterophylla; and 1.67, 1.10 and 1.32 μg/ml for A. bidwillii, respectively. Caspase-3 activation indicated that the cytotoxicity of A. bidwillii essential oil is mediated via caspase-dependent apoptosis. These findings suggest that the essential oils of A. heterophylla and A. bidwilli offer promising anticancer drug candidates pertaining to the synergistic effects of their individual components.

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Hepatoprotection and Antioxidant Activity of Gazania longiscapa and G. rigens with the Isolation and Quantitative Analysis of Bioactive Metabolites - 01/0

Wafaa Mostafa Hussein Ali Elkady

Samar Y Desoukey, Wafaa M El Kady, Abeer A A Salama, Eman G Hagag, Siham M El-Shenawy, M A El-Shanawany

01/07/2016

Gazania longiscapa and G. rigens are two species belonging to family Asteraceae. The present study aimed the isolation of the main active constituents from the methanol extracts using different chromatographic methods and their identification using different spectroscopic techniques, beside the quantitation of some biologically important active constituent as rutin using HPLC technique, together with estimation of total polyphenolic content calculated as gallic acid and estimation of total flavonoid content calculated as rutin using UV technique. Concomitantly the determination of the antioxidant and hepatoprotective activity of the total methanol extracts of the aerial parts of G. longiscapa and G. rigens. This work resulted in the isolation of 4 flavonoids (Apigenin, Luteolin, Luteolin 7-O-β-D-glucopyranosid, Apigenin 7-O-β-D-glucopyranosid), 3 phenolic acids (Caffeic acid, Chlorogenic acid and 3,5- di- O-caffeoylquinic acid) from G. longiscapa for the first time; these 3 phenolic acids were also isolated from G. rigens, together with one flavonoid (rutin), The quantitative determination of the methanol extracts showed that G. longiscapa is a richer source of phenolic acids than G. rigens and both Gazania species are valuable sources of rutin beside having hepatoprotective and antioxidant activity.

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Comparative DNA profiling, botanical identification and biological evaluation of Gazania longiscapa DC and Gazania rigens L. - 01/1

Wafaa Mostafa Hussein Ali Elkady

Wafaa M. El Kady, Abeer A.A. Salama , Samar Y. Desoukey,Eman G. Hagag, Siham M. El-Shenawy, M.A. El-Shanawany

01/12/2015

Abstract Gazania longiscapa DC and Gazania rigens L. are species of cultivated ornamental plant that grow in Egypt. Genus Gazania has a role in folk medicine to prevent toothache; this study presents a comparative investigation of genetic and botanical features of root, rhizome, leaves and flowers of the two Gazania species and comparing their biological activity as analgesic and antiinflammatory as related to their folk medicinal use. The genetic and botanical differences between the two Gazania species are reported for the first time in this study. The results contribute toward validation of the traditional use of Gazania showing that both species are safe for oral administration and they exhibit significant antinociceptive and anti-inflammatory effects in a dose dependent manner.

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Polyphenolic Profile And Biological Activity Of Salvia Splendens Leaves - 01/0

Wafaa Mostafa Hussein Ali Elkady

Fatma Abd-elkader Moharram, Mohamed Soubhi Marzouk, Siham Mustafa El-Shenawy and Wafaa Mostafa El Kady

01/01/2012

Objectives: The aim of this study was to investigate a new flavone triglycoside, together with eleven phenolic metabolites from 80% aqueous methanol extract of S. Splendens leaves (AME) and assessment of its hypoglycemic and anti-inflammatory activities along with in vitro antioxidant effect. Methods: The phenolic composition of S. Splendens leaves was analyzed using UV, 1D and 2D NMR and negative ESI-MS spectroscopy. Hypoglycemic activity of AME was assessed by measuring blood glucose in streptozotocin induced diabetic rats. Key findings: Twelve phenolic metabolites including three phenolic acids, namely cafeeic acid 1, rosmarinic acid 2 and methyl rosmarinate 3, four flavone glycosides viz the new compound luteolin. Moreover, AME exhibited a significant anti-inflammatory activity only at 100 mg/kg in comparison to indomethacin. Finally, AME exhibited a marked significant scavenging activity against DPPPH, the maximum reactive reaction rate after 5 min was 62.9, 82.5, 83.7, 84.3 and 85.1 % for the concentration 10, 20, 30, 40 and 50 mg/ml, respectively. Conclusions: This is the first study reporting the identification of a new flavone along with eleven known phenolic metabolites from AME of S. Splendens. It showed significant hypoglycemic and anti-inflammatory effects in dose dependent manner. Moreover, it showed an in vitro antioxidant activity.

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