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Ramzia Ibrahim

Basic information

Name : Ramzia Ibrahim
Title: Professor of Pharmaceutical chemistry, Head of Pharmaceutical Chemistry Department.
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Personal Info: Professor Ramzia Ibrahim, professor of Pharmaceutical chemistry - Department of Pharmaceutical Chemistry, Head of Pharmaceutical Chemistry Department. She has a PH.D and MSC degree in Pharmaceutical Chemistry from Cairo university. View More...

Education

Certificate Major University Year
PhD Pharmaceutical sciences - Pharmaceutical Chemistry Cairo University 1990
Masters Pharmaceutical sciences - Pharmaceutical Chemistry Cairo University 1986
Bachelor Pharmaceutical Sciences & Pharmaceutical Industries Cairo University 1980

Teaching Experience

Name of Organization Position From Date To Date
Cairo University Professor 01/01/2008 01/01/2010
Cairo University Associate Professor 01/01/2002 01/01/2008
Cairo University Lecturer 01/01/1990 01/01/2002
Cairo University Assistant Lecturer 01/01/1986 01/01/1990
Cairo University Demonstrator 01/01/1980 01/01/1986

Researches /Publications

Quantitative Nuclear Magnetic Resonance Spectroscopic Analysis of Two Commonly Used Gastrointestinal Tract Drugs. - 01/0

Ramzia Ismail Ibrahim El Bagary

Fouad MA, Ezzeldin MI.

01/03/2020

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Determination of some antiemetic drugs through its native fluorescence or fluorescence quenching of cerrous ammonium sulphate. - 01/0

Ramzia Ismail Ibrahim El Bagary

Hassib ST, Taha EA, Barakat GH

01/03/2020

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Simultaneous Determination of Meclizine Hydrochloride in Its Mixtures with Pyridoxine Hydrochloride, Caffeine or Nicotinic Acid Using HPLC and TLC-Densitometric Methods.2020 - 01/1

Ramzia Ismail Ibrahim El Bagary

Hassib ST, Taha EA, Barakat GH.

01/12/2019

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Novel spectrophotometric methods for the determination of Leflunomide and Diacerein in binary mixtures. - 01/0

Ramzia Ismail Ibrahim El Bagary

Mahrouse MA, El-Hakeem MM, Megally AB, Mohamed AA.

01/09/2019

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Novel Spectrophotometric methods for the determination of Leflunomide and Diacerin in binary mixtures - 01/0

Ramzia Ismail Ibrahim El Bagary

Marianne A. Mahrouse, Maha M. El-Hakeem, Amal B. Megally, Amira A. Mohamed

01/09/2019

Two novel spectrophotometric methods were presented in this work using ethanol as a solvent. The first method was the ratio difference spectrophotometric method [RDSM], in which the amplitude difference between two selected wavelengths on the ratio spectra were recorded and used for estimation of each of Leflunomide LEF in mixture with its alkaline induced degradate DEG and also for Diacerein DIA determination in mixture with Aceclofenac ACEC without interference from the other component in the mixture. The second method is the ratio subtraction coupled with constant multiplication [RS-CM], where LEF was determined in its mixture with its alkaline degradate DEG at 261 nm which is considered as a stability indicating assay. In addition to simultaneous determination of Diacerein DIA and Aceclofenac ACEC in their mixtures at 257 and 277 nm, respectively, by the second method without previous separation. Linearity was shown over the concentration range of [1.5-15 μg/ml] for LEF, [1-11 μg/ml] for DIA and [2.5-25 μg/ml] for ACEC, by both proposed methods. Leflunomide was found to be completely degraded when subjected to alkaline degradation producing one alkaline product. Validation of the suggested methods was conducted according to ICH guidelines, concerning precision, accuracy, repeatability. The suggested spectrophotometric methods were statistically compared to reference methods showing no significant difference. The suggested spectrophotometric methods are considered to be simple, sensitive and could be easily applied in quality control laboratories instead of LC methods.

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STABILITY STUDY AND VALIDATED REVERSED PHASE LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF TIROFIBAN HYDROCHLORIDE IN PRESENCE OF TYROSINE AS A PROCESS IMPURITY - 01/0

Ramzia Ismail Ibrahim El Bagary

Ehab F. Elkady, Naira A. Farid, Nadia F. Youssef

01/06/2018

Tirofiban hydrochloride was subjected to the degradation under conditions of hydrolysis (acidic and alkaline degradation), oxidative, thermal and photolytic degradation as prescribed by ICH. A simple and precise liquid chromatographic method has been developed and validated for the simultaneous determination of tirofiban hydrochloride monohydrate (TIR) and its synthetic starting material; tyrosine (TRS). All the chromatographic separations were achieved on Zorbax SB C18, 250 mm×4.6 mm i.d., 5μm column at a flow rate of 1 mL min–1. Isocratic elution based on 0.1 M phosphate buffer (pH 3) - acetonitrile (70:30, v/v) with UV detection at 227 nm was applied. For the stability study separation of TIR from its degradation products was achieved using 0.1 M phosphate buffer (pH 3) - acetonitrile (72:28, v/v) with UV detection at 210 nm. Method validation parameters namely, linearity, accuracy and precision were found to be acceptable over the concentration ranges of 10-250 μg mL–1 for TIR and 1-70 μg mL–1 for TRS. The minimum detection limits were 1.76 μg mL–1 for TIR and 0.13 μg mL–1 for TRS. The optimized method was validated and proved to be specific, robust and accurate for the quality control of the cited drug in drug substance and drug product

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A novel method for determination of tinidazole and metronidazole in aqueous solutions based on fluorescence quenching of functionalized CdS quantum dots as luminescent probes - 01/0

Ramzia Ismail Ibrahim El Bagary

Asmaa A El-Zaher, Ehab Elkady,

01/05/2018

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Validated spectrofluorimetric methods for the determination of apixaban and tirofiban hydrochloride in pharmaceutical formulations. - 01/0

Ramzia Ismail Ibrahim El Bagary

Ehab F. Elkady, Naira A. Farid, Nadia F. Youssef

01/03/2017

Apixaban and Tirofiban Hydrochloride are low molecular weight anticoagulants. The two drugs exhibit native fluorescence that allow the development of simple and valid spectrofluorimetric methods for the determination of Apixaban at λ ex/λ em = 284/450 nm and tirofiban HCl at λ ex/λ em = 227/300 nm in aqueous media. Different experimental parameters affecting fluorescence intensities were carefully studied and optimized. The fluorescence intensity-concentration plots were linear over the ranges of 0.2–6 μg ml− 1 for apixaban and 0.2–5 μg ml− 1 for tirofiban HCl. The limits of detection were 0.017 and 0.019 μg ml− 1 and quantification limits were 0.057 and 0.066 μg ml− 1 for apixaban and tirofiban HCl, respectively. The fluorescence quantum yield of apixaban and tirofiban were calculated with values of 0.43 and 0.49. Method validation was evaluated for linearity, specificity, accuracy, precision and robustness as per ICH guidelines. The proposed spectrofluorimetric methods were successfully applied for the determination of apixaban in Eliquis tablets and tirofiban HCl in Aggrastat intravenous infusion. Tolerance ratio was tested to study the effect of foreign interferences from dosage forms excipients. Using Student's t and F tests, revealed no statistically difference between the developed spectrofluorimetric methods and the comparison methods regarding the accuracy and precision, so can be contributed to the analysis of apixaban and tirofiban HCl in QC laboratories as an alternative method.

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A concise comparative mini review between HPLC-UV and spectrophotometric analysis of gliptins in pharmaceutical formulations. - 01/0

Ramzia Ismail Ibrahim El Bagary

Shereen Mowaka, Moataz S Hendy, Mohamed M Elmazar, Andrew H Hakeem, Ehab F Elkady, Mirna M Antoun, Mai M Shaalan, Sara A Abdel-Wahab, Mai M Elsemeiri, Nourhan K Abdelaziz, Alaa E Mohamed, Menna H Eltahawy, Asmaa M Rashid, Rola M Emam, Mahmoud M Youssef, Muhammad N El-Kattan, Marwa A Sayed, Ahmed M Kowider, Adly H Seha, Engy A Rabea, Rana M Yakout, Rolly H Faried, Bassam M Ayoub

01/01/2017

The ongoing development of anti-diabetic drugs brings a revolution in the treatment of diabetes mellitus. Dipeptidyl Peptidase-4 (DPP-4) inhibitors are considered a new class of oral anti-diabetic agents used in treatment of type 2 diabetes mellitus. Therefore, the necessity to explore and compare the existing analytical method used for estimation of such drugs either single or in combination is crucial. This review offers an overview of different HPLC-UV and spectrophotometric methods used for determination of DPP-4 inhibitors namely; sitagliptin, vildagliptin, saxagliptin, linaglitpin and alogliptin in a tabulated comparative way. In addition, the present work included stability indicating assays of the drugs and determination of their process related impurities. Spectrophotometric assays showed more facilitated, simple and cost effective methods than the reported chromatographic techniques. Furthermore, the reviewed spectrophotometric methods showed the advantages of low cost solvents, shorter analysis time and simple instrumentation instead of complex details implemented in the chromatographic method development. The developed comparative review should be of interest to the analysts in the area of drug control and can be used by quality control laboratories for the recently approved gliptin combinations.

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Stability-Indicating RP-HPLC Methods for the Determination of Fluorometholone in Its Mixtures with Sodium Cromoglycate and Tetrahydrozoline Hydrochloride - 01/0

Ramzia Ismail Ibrahim El Bagary

Fouad MA2, El-Shal MA3, Tolba EH3

01/07/2016

Two stability-indicating reversed-phase liquid chromatographic methods were developed and validated for the determination of fluorometholone (FLU) in its mixtures with sodium cromoglycate (SCG) and tetrahydrozoline hydrochloride (THZ). The first HPLC method (Method 1) was based on isocratic elution of FLU and SCG along with their alkaline degradation products on a reversed phase C18 column (250 × 4.6 mm id)-ACE Generix 5, using a mobile phase consisting of methanol-water (70 : 30, v/v), pH adjusted to 2.5 using orthophosphoric acid at a flow rate of 1.2 mL min(-1) Quantitation was achieved with UV detection at 240 nm. The second HPLC method (Method 2) was based on isocratic elution of FLU, its alkaline degradation product and THZ on a reversed phase C8 column (250 × 4.6 mm)-ACE Generix 5, using a mobile phase consisting of acetonitrile-50 mM potassium dihydrogen orthophosphate (40 : 60, v/v) at a flow rate of 2 mL min(-1) Quantitation was achieved by applying dual-wavelength detection, where FLU and its alkaline degradation product were detected at 240 nm and THZ was detected at 215 nm at ambient temperatures. Linearity, accuracy and precision were found to be acceptable over the concentration range of 5-50 and 10-500 μg mL(-1) for FLU and SCG (Method 1) and over the concentration range of 5-80 and 5-60 μg mL(-1) for FLU and THZ (Method 2), respectively. Besides, the FLU alkaline degradation product was verified using IR, NMR and LC-MS spectroscopy. The two proposed methods could be successfully applied for the routine analysis of the studied drugs either in their pure bulk powders or in their pharmaceutical preparations without any preliminary separation step.

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UPLC–MS-MS Method for the Determination of Vilazodone in Human Plasma: Application to a Pharmacokinetic Study - 01/0

Ramzia Ismail Ibrahim El Bagary

Hashem H, Fouad M, Tarek S

01/05/2016

A sensitive, rapid and simple liquid chromatographic-electrospray ionization tandem mass spectrometric (LC-ESI-MS-MS) method was developed for the quantitative determination of vilazodone in human plasma and for the study of the pharmacokinetic behavior of vilazodone in healthy Egyptian volunteers. With escitalopram as internal standard (IS), liquid-liquid extraction was used for the purification and preconcentration of analytes from human plasma matrix using diethyl ether. The separation was performed on an Acquity UPLC BEH shield RP C18 column (1.7 µm, 2.1 × 150 mm). Isocratic elution was applied using methanol-0.2% formic acid (90:10, v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with multiple reaction monitoring mode via an electrospray ionization source at m/z 442.21 → 155.23 for vilazodone and m/z 325.14 → 109.2 for escitalopram. Linear calibration curves were obtained over the range of 1-200 ng/mL with the lower limit of quantification at 1 ng/mL. The intra- and inter-day precision showed relative standard deviation ≤3.3%. The total run time was 1.5 min. This method was successfully applied for clinical pharmacokinetic investigation, and a preliminary metabolic study was also carried out.

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Simultaneous Determination of Metformin, Vildagliptin and 3-amino-1-adamantanol in Human Plasma: Application to Pharmacokinetic Studies - 01/0

Ramzia Ismail Ibrahim El Bagary

Hassan M. E. Azzazyb, Ehab F. ElKadyc & Faten Faroukd*

01/04/2016

Metformin (MET) and vildagliptin (VLD) are coformulated in tablets for the management of diabetes mellitus. The aim of this study is the development of a new fast ultraperformance liquid chromatography method with tandem mass detection (UPLC–MS/MS) for their simultaneous determination with 3-amino-1-adamantanol (starting compound for vildagliptin synthesis; VLI) in human plasma. Separation of MET, VLD, and VLI was performed on a 5 cm UPLC-C18 column using a mobile phase of 0.5% acetic acid in methanol and 0.02 M aqueous ammonium acetate (10:90, v/v). The injection volume was 10 µL and electrospray positive ionization was applied. Extraction from human plasma was carried out by acid precipitation of plasma proteins using pregabalin as an internal standard. The assay was validated according to ICH guidelines. The developed method is valid, fast, and simple and was successfully applied in pharmacokinetic studies in human volunteers.

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Simultaneous determination of ciprofloxacin hydrochloride and metronidazole in spiked human plasma by ultra performance liquid - 01/0

Ramzia Ismail Ibrahim El Bagary

Asmaa Ahmed El-Zaher, Ehab Elkady, Asmaa Abdelkreem

01/03/2016

Ciprofloxacin HCl (CIP) and Metronidazole (MET) are antibacterial drugs used in combination for treatment of mixed aerobic/anaerobic infections. An UPLC-MS/MS method was developed for the simultaneous estimation of CIP and MET in spiked human plasma using sildenafil citrate as an internal standard (IS). Protein precipitation was used for analyte extraction. The chromatographic separation was completed within 6 min using a mobile phase of 0.1% formic acid in water and acetonitrile (70: 30, v/v), Zorbax C18, 100 x 4.6 mm, 3.5 µm analytical column, at a flow rate of 0.5 mL min-1 . Multiple reaction monitoring (MRM) transitions were measured in the positive ion mode. Validation of the method showed standard curves to be linear in the range of 10-4000 ng mL-1 for CIP and 30-12000 ng mL-1 for MET with mean correlation coefficient exceeding 0.999. In human plasma, CIP and MET were stable for at least 36 days at –70 ± 5 °C, 6 hours at ambient temperature and after three freeze thaw cycles. After extraction from plasma, the samples were stable in auto sampler at 22 °C for 6 hours. The method was simple, specific, sensitive, precise, accurate and suitable for bioequivalence and pharmacokinetic studies.

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Novel liquid chromatographic methods for the determination of varenicline tartarate - 01/0

Ramzia Ismail Ibrahim El Bagary

Nisreen F. Abo- Talib , Marwa A. El- Wahab

01/01/2016

Two simple, sensitive, rapid, and stability-indicating liquid chromatographic (LC) methods have been developed for the determination of varenicline tartrate. They comprised the determination of varenicline (VRC) in the presence of its oxidative degradates and related impurity (N-formyl varenicline) (NFV). The first method was a LC with diode array detection (DAD) at 235 nm using Ristek-Ultras C18 column (100 mm  2.1 mm, 5 mm). Isocratic elution of VRC was employed using a mobile phase consisting of buffer mixture (1.2% potassium dihydrogen phosphate and 0.08% octane sulphonic acid): acetonitrile (86:14, v/v), pH (5.0). In the second method; a fluorimetric detection technique was developed, based on precolumn derivatization of VRC using 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-Cl). The fluorescence detector (FLD) was operated at 474 nm for excitation and 539 nm for emission. Isocratic elution was applied with a mobile phase consisting of methanol-distilled water (70:30, v/v). Separation was achieved using Symmetrys Waters C18 column (150 mm  4.6 mm, 5 mm). Linearity, accuracy and precision were found to be acceptable over the concentration ranges of 0.5–20.0 mg mL1 and 0.2– 20.0 mg mL1 with the first and the second method, respectively. The optimized methods were validated and proved to be specific, simple, and accurate for the quality control of the drug in its pharmaceutical preparation. & 2015

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Stability-indicating RP-LC method for determination of azilsartan medoxomil and chlorthalidone in pharmaceutical dosage forms: application to degradation kinetics - 01/0

Ramzia Ismail Ibrahim El Bagary

Walid A. Ebeid, Ehab F. Elkady, Asmaa A. Elzahr,, Gabor Patonay

01/01/2015

A RP-LC method was developed and validated for simultaneous determination of the active components, azilsartan medoxomil (AZL) and chlorthalidone (CLT), in their novel antihypertensive combined recipe. The chromatographic separation was achieved on an Eclipse XDB-C18 (4.6 × 150 mm, 5 μm) column using a mobile phase consisting of methanol/potassium hydrogen phosphate buffer (pH 8, 0.05 M) (40:60, v/v) in isocratic mode. The flow rate was maintained at 0.8 mL min−1 at ambient temperature. Detection was carried out at 210 nm. The method was validated according to the ICH guidelines. Linearity, accuracy, and precision were satisfactory over the concentration range of 5.0–50.0 and 2.5–25.0 μg mL−1 for AZL and CLT, respectively (r 2 = 0.9999). LODs for AZL and CLT were 0.90 and 0.32 μg mL−1, whereas LOQs were 2.72 and 0.98 μg mL−1, respectively. Both drugs were subjected to forced degradation studies under hydrolysis (neutral, acidic, and alkaline), oxidative, and photolytic extensive stress conditions. The proposed method is stability indicating by the resolution of the investigated drugs from their degradation products. Moreover, the kinetics of the acidic degradation of AZL as well as the kinetics of the alkaline degradation of CLT were investigated. Arrhenius plots were constructed and the apparent first-order rate constants, half-life times, shelf-life times, and the activation energies of the degradation processes were calculated. The method was successfully applied for the determination of the studied drugs simultaneously in their coformulated tablet. The developed method is specific and stability indicating for the quality control and routine analysis of the cited medications in their pharmaceutical preparations

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Validated HPLC and Ultra-HPLC Methods for Determination of Dronedarone and Amiodarone–Application for Counterfeit Drug Analysis - 01/0

Ramzia Ismail Ibrahim El Bagary

Elkady, Ehab2; Mowaka, Shereen3; Attallah, Maria4

01/01/2015

Two simple, accurate, and precise chromatographic methods have been developed and validated for the determination of dronedarone (DRO) HCl and amiodarone (AMI) HCl either alone or in binary mixtures due to the possibility of using AMI as a counterfeit of DRO because of its lower price. First, an RP-HPLC method is described for the simultaneous determination of DRO and AMI. Chromatographic separation was achieved on a BDS Hypersil C18 column (150 × 4.6 mm, 5 μm). Isocratic elution based on potassium dihydrogen phosphate buffer with 0.1% triethylamine pH 6–methanol (10 + 90, v/v) at a flow rate of 2 mL/min with UV detection at 254 nm was performed. The second method is RP ultra-HPLC in which the chromatographic separation was achieved on an AcclaimTM RSLC 120 C18 column (100 × 2.1 mm, 2.2 μm) using isocratic elution with potassium dihydrogen phosphate buffer with 0.1% triethylamine pH 6–methanol (5 + 95, v/v) at a flow rate of 1 mL/min with UV detection at 254 nm. Linearity, accuracy, and precision of the two methods were found to be acceptable over the concentration ranges of 5–80 μg/mL for both DRO and AMI. The results were statistically compared using one-way analysis of variance. The optimized methods were validated and proved to be specific, robust, precise, and accurate for the QC of the drugs in their pharmaceutical preparations.

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Stability Indicating HPLC Method for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in the Presence of Ciprofloxacin Acid Degradation Product. - 01/0

Ramzia Ismail Ibrahim El Bagary

Asmaa A. El-Zaher, Ehab F. Elkady, Maha M. El-Hakim, Asmaa Mandoor

01/01/2015

A stability indicating HPLC method for the simultaneous determination of ciprofloxacin HCl (CIP) and metronidazole (MET) in presence of ciprofloxacin acid degradation product (DEG) is presented (Method I, MI). The present study is concerned with the development and validation of an environmentally friendly method with relatively low organic composition of the mobile phase. The chromatographic separation of the two drugs was performed using Kromasil 100-5C18 (150 mm x 4.6 mm) with UV detection at 280 nm and flow rate of 1 mLmin-1. The mobile phase consisted of 0.5% aqueous phosphoric acid and acetonitrile (90:10 v/v) MI. In addition, another HPLC method (MII) for the separation of the same binary mixture using 0.5% aqueous phosphoric acid and acetonitrile (80:20 v/v) was presented. Retention times were 2.40, 3.10 & 22.94 min for MET, DEG and CIP, respectively, MI. On the other hand, the retention times were 2.03 and 4.00 min for MET and CIP, respectively MII. However, the relatively low organic composition of the mobile phase of 10% in MI was advantageous regarding green analytical procedure concept. Linearity, accuracy and precision were acceptable over the concentration range of 1-65 μgmL-1 for both drugs MI, and 1-80 μgmL-1 MII. The method is new, simple , sensitive and suitable for the routine quality control and dosage form assay of both drugs in the presence of the acid degradation product of ciprofloxacin. The method showed sufficient accuracy with a mobile phase of only 10% organic composition showing advantage and trying to approach green chromatographic conditions.

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Steady-state and synchronous spectrofluorimetric methods for simultaneous determination of aliskiren hemifumarate and amlodipine besylate in dosage forms - 01/0

Ramzia Ismail Ibrahim El Bagary

Walid M. Ebeid,Ehab F. Elkady,Asmaa A. El-Zaher, Gabor Patonay

01/01/2014

Aliskiren hemifumarate (ALS) and amlodipine besylate (AML) were simultaneously determined by two different spectrofluorimetric techniques. The first technique depends on direct measurement of the steady-state fluorescence intensities of ALS and AML at 313 nm and 452 nm upon excitation at 290 and 375 nm, respectively, in a solvent composed of methanol and water (10: 90, v/v) . The second technique utilizes synchronous fluorimetric quantitative screening of the emission spectra of ALS and AML at 272 and 366 nm, respectively using Δλ of 97 nm. Effects of different solvents and surfactants on relative fluorescence intensity were studied. The method was validated according to ICH guidelines. Linearity, accuracy and precision were found to be satisfactory in both techniques over the concentration ranges of 1–15 and 0.4–4 µg/mL for ALS and AML, respectively. In the first technique, limit of detection and limit of quantification were estimated and found to be 0.256 and 0.776 µg/mL for ALS as well as 0.067 and 0.204 µg/mL for AML, respectively. Also, limit of detection and limit of quantification were calculated in the synchronous method and found to be 0.293 and 0.887 µg/mL for ALS as well as 0.034 and 0.103 µg/mL for AML, respectively. The methods were successfully applied for the determination of the two drugs in their co-formulated tablets. The results were compared statistically with reference methods and no significant difference was found. The developed methods are rapid, sensitive, inexpensive and accurate for the quality control and routine analysis of the cited drugs in bulk and in pharmaceutical preparations without pre-separation. Copyright © 2014 John Wiley & Sons, Ltd.

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Application of chromatographic and spectrophoitometric methods for the analysis of selected antihypertensive compinations - 01/0

Ramzia Ismail Ibrahim El Bagary

Ezzeidin, M. I. , Shokry, E. , Fouad, M. A.

01/03/2013

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Simultaneous determination of Metformin, Vildagliptin, and Vildagliptin impurity in bulk, tablet, and human plasma using UPLC-MS/MS - 01/0

Ramzia Ismail Ibrahim El Bagary

El Kady, EF; Farouk, F; Azzazy, HME

01/01/2013

Metformin (M) and vildagliptin (V) are co-administered in tablets used for the management of patients with type II diabetes mellitus. We have developed an UPLC-MS/MS method for simultaneous determination of M, V, and vildagliptin impurity (VI) in bulk, tablet, and plasma. The method employed electro-spray positive ionization and multiple-reaction monitoring mode. The m/z values for M, V, and VI were 130: 71, 60; 304: 154, 97; and 168: 151, 93.1 [M+ H]+; respectively. Chromatographic elution was achieved in 2 min on an UPLC C-18 ...

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Application of Chromatographic and Spectrophotometric Methods for The Analysis of Aliskiren and Hydrochlorothiazide Antihypertensive Combination - 01/0

Ramzia Ismail Ibrahim El Bagary

Menna I. Ezzeldin, Engy Shokry, Marwa Fouad

01/01/2013

High performance liquid chromatographic (HPLC) and spectrophotometric methods developed for the simultaneous determination of Aliskiren (ALK) and Hydrochlorothiazide (HCT) combination in bulk powder and in tablets dosage form. Determination of ALK and HCT was achieved by chromatographic separation on Econosphere C-18 column using a mobile phase consisting of water (pH 7.5): acetonitrile (50:50) at a flow rate of 0.5 mL.min−1 and UV detection at 208 nm. Method validation parameters were found to be acceptable over the concentrations range of 5-150 µg.mL-1 , 1-50 µg.mL-1 for ALK and HCT respectively. Regarding the spectrophotometric methods, two methods were employed. Simultaneous Equation method, absorbance readings are taken at two wavelengths 277.48 nm (for ALK) and 267.48 nm (for HCT) in methanol. Dual Wavelength method, the difference between absorbance readings are taken at two wavelengths 273.3 nm, 260 nm (for Aliskiren) and 270 nm, 280 nm (for HCT) in methanol. The applied spectrophotometric methods were found to be rapid, specific, precise and accurate over the concentration range of 5 – 150 µg.mL-1 and 1 – 41 µg.mL-1 for ALK and HCT respectively and can be applied for the routine analysis of these drugs in bulk, and combined dosage form without any interference by the excipients.

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Three Validated Methods Of Simultaneous Determination Of Ofloxacin And Dexamethasone In Binary Mixture - 01/0

Ramzia Ismail Ibrahim El Bagary

01/01/2013

Three selective precise and accurate methods were developed and validated for the simultaneous determination of ofloxacin and dexamethasone in bulk powder and pharmaceutical formulation, namely, derivative ratio spectrophotometric, denistometric and HPLC method. The first method is based on the use of derivative ratio spectrophotometric technique which allows the determination of ofloxacin and dexamethasone at 286,235 nm over concentration range 1-13.5 and 1-7 g mL for ofloxacin and dexamethasone, respectively. The second method is denistometric one which depended on the separation of silica gel plate using methanol-phosphate buffer (4:6 v/v) and pH adjusted to 5 with orthophoshoric acid as a developing system and the spots were scanned at 300 and 240 nm for ofloxacin and dexamethasone, respectively. The last method is HPLC using acetonitrile – H2o (7:3, v/v) and pH adjusted to 6.08 as the mobile phase at a flow rate of 1.5 mL/min and UV detection at 254 nm. The suggested methods were successfully applied for simultaneous determination of floxacin and dexamethasone in bulk, laboratory prepared mixtures and pharmaceutical dosage form with good accuracy and precision. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by the official methods.

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