Future University In Egypt (FUE)
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Altagamoa Al Khames, Main centre of town, end of 90th Street
New Cairo
Egypt

Sara Zahran

Basic information

Name : Sara Zahran
Title: Lecturer
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Personal Info: Sara Ahmed, Msc, Assistant Lecturer in Microbiology and Immunology department; MSc in Microbiology and Immunology, Cairo University; BSc in pharmacy, Ain Shams University. View More...

Education

Certificate Major University Year
PhD Microbiology and Immunology Cairo University-Faculty of Pharmacy 2019
Masters Microbiology and Immunology Cairo University 2013
Bachelor Pharmaceutical Sciences Ain Shams University - Faculty of Pharmacy 2003

Teaching Experience

Name of Organization Position From Date To Date
MIU Assistant Lecturer 01/01/2008 01/01/2011

Researches /Publications

Azoreductase activity of dye-decolorizing bacteria isolated from the human gut microbiota - 01/0

Sara Ahmed Zahran Mohamed Awad

Abdelgawad M. Hashem , Ramy K. Aziz

01/04/2019

The gut microbiota enriches the human gene pool and contributes to xenobiotic metabolism. Microbial azoreductases modulate the reduction of azo-bonds, activating produgs and azo polymer-coated dosage forms, or degrading food additives. Here, we aimed to screen the healthy human gut microbiota for food colorant-reducing activity and to characterize factors modulating it. Four representative isolates from screened fecal samples were identified as E. coli (AZO-Ec), E. faecalis (AZO-Ef), E. avium (AZO-Ev) and B. cereus (AZO-Bc). Both AZO-Ef and AZO-Ev decolorized amaranth aerobically and microaerophilically while AZO-Ec and AZO-Bc had higher aerobic reduction rates. The isolates varied in their activities against different dyes, and the azo-reduction activity mostly followed zero-order reaction kinetics, with a few exceptions. Additionally, the isolates had different pH dependence, e.g., AZO-Ec was not affected by pH variation while AZO-Bc exhibited variable degradation kinetics at different pH levels. Cell-free extracts showed NADH-dependent enzymatic activities 14–19 times higher than extracellular fractions. FMN did not affect the reducing activity of AZO-Ef cell-free extract, whereas AZO-Ec, AZO-Ev and AZO-Bc had significantly higher reduction rates in its presence (P values = 0.02, 0.0001 and 0.02, respectively). Using Degenerate primers allowed the amplification of azoreductase genes, whose sequences were 98–99% similar to genes encoding FMN-dependent-NADH azoreductases.

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