Future University In Egypt (FUE)
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Asmaa Mandour

Basic information

Name : Asmaa Mandour
Title: Lecturer of Pharmaceutical chemistry
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Personal Info: Dr. Asmaa Abdelkereim Mandour, Lecturer of Pharmaceutical chemistry - Department of Pharmaceutical chemistry. She has a PH.D and MSC degree in Pharmaceutical Chemistry from Cairo university View More...


Certificate Major University Year
PhD Pharmaceutical Chemistry Faculty of Pharmaceutical - Cairo University 2015
Masters Pharmaceutical Chemistry Cairo University 2010
Bachelor Faculty Of Pharmacy Ain Shams University 2000

Teaching Experience

Name of Organization Position From Date To Date
Bioequivalence Center (DRC) Quality Control Manager 01/01/2011 01/01/2012
Misr International University Teaching Assistant/ Assistant Lecturer, Pharmaceutical Chemistry Department 01/09/2001 01/10/2010
6th October University Teaching Assistant, Pharmacognocy and Midicinal Plants 01/02/2001 01/05/2001

Researches /Publications

Sequential liquid-liquid extraction coupled to LC-MS/MS for simultaneous determination of amlodipine, olmesartan and hydrochlorothiazide in plasma samples: Application to pharmacokinetic studies. - 01/0


Elkady EF, Algethami FK, Aboelwafa AA, Farouk F.


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Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in spiked Human Plasma by Ultra Performance Liquid Chromatography- Tandem Mass Spectroscopy - 01/0


Ramzia El-Bagary, Asmaa Ahmed El-Zaher, Ehab Elkady


Ciprofloxacin HCl (CIP) and Metronidazole (MET) are antibacterial drugs used in combination for treatment of mixed aerobic/anaerobic infections. An UPLC-MS/MS method was developed for the simultaneous estimation of CIP and MET in spiked human plasma using sildenafil citrate as an internal standard (IS). Protein precipitation was used for analyte extraction. The chromatographic separation was completed within 6 min using a mobile phase of 0.1% formic acid in water and acetonitrile (70: 30, v/v), Zorbax C18, 100 x 4.6 mm, 3.5 μm analytical column, at a flow rate of 0.5 mL min-1. Multiple reaction monitoring (MRM) transitions were measured in the positive ion mode. Validation of the method showed standard curves to be linear in the range of 10-4000 ng mL-1 for CIP and 30-12000 ng mL-1 for MET with mean correlation coefficient exceeding 0.999. In human plasma, CIP and MET were stable for at least 36 days at –70 ± 5 °C, 6 hours at ambient temperature and after three freeze thaw cycles. After extraction from plasma, the samples were stable in auto sampler at 22 °C for 6 hours. The method was simple, specific, sensitive, precise, accurate and suitable for bioequivalence and pharmacokinetic studies.

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Stability Indicating HPLC Method for the Simultaneous Determination of Ciprofloxacin - 01/0


Ramzia I. El-Bagary, Asmaa A. El-Zaher, Ehab F. Elkady, Maha M. El-Hakim


A stability indicating HPLC method for the simultaneous determination of ciprofloxacin HCl (CIP) and metronidazole (MET) in presence of ciprofloxacin acid degradation product (DEG) is presented (Method I, MI). The present study is concerned with the development and validation of an environmentally friendly method with relatively low organic composition of the mobile phase. The chromatographic separation of the two drugs was performed using Kromasil 100-5C18 (150 mm x 4.6 mm) with UV detection at 280 nm and flow rate of 1 mLmin-1. The mobile phase consisted of 0.5% aqueous phosphoric acid and acetonitrile (90:10 v/v) MI. In addition, another HPLC method (MII) for the separation of the same binary mixture using 0.5% aqueous phosphoric acid and acetonitrile (80:20 v/v) was presented. Retention times were 2.40, 3.10 & 22.94 min for MET, DEG and CIP, respectively, MI. On the other hand, the retention times were 2.03 and 4.00 min for MET and CIP, respectively MII. However, the relatively low organic composition of the mobile phase of 10% in MI was advantageous regarding green analytical procedure concept. Linearity, accuracy and precision were acceptable over the concentration range of 1-65 μgmL-1 for both drugs MI, and 1-80 μgmL-1 MII. The method is new, simple , sensitive and suitable for the routine quality control and dosage form assay of both drugs in the presence of the acid degradation product of ciprofloxacin. The method showed sufficient accuracy with a mobile phase of only 10% organic composition showing advantage and trying to approach green chromatographic conditions.

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