Future University In Egypt (FUE)
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Altagamoa Al Khames, Main centre of town, end of 90th Street
New Cairo
Egypt

Osama Salama

Basic information

Name : Osama Salama
Title: Professor & University Vice President
Personal Info: Prof. Dr. Osama Salama is the Vice President for student affairs.

Education

Certificate Major University Year
PhD Pharmacy Science - drugs ETH Zurich, Switzerland 1982
Diploma نباتات طبية Assistant II, Pharmazeutisches Institut 1979
Masters Pharmacognosy Assuit University 1973
Bachelor Pharmacy and Pharmaceutical Chemistry Alexandria University 1967

Teaching Experience

Name of Organization Position From Date To Date
Future University in Egypt Dean Faculty of Pharmaceutical Sciences 01/01/2007 01/01/2010
Pharco Pharmaceuticals, Alex., Egypt Res., Dev & Q.C. Divisions Director 01/01/1996 01/01/2007
Mansoura University. Prof. in Pharmacognosy and Phytochemistry, Faculty of Pharmacy, 01/01/1991 01/01/2002
Mansoura University Assoc. Prof. in Pharmacognosy and Phytochemistry, Faculty of Pharmacy, 01/01/1986 01/01/1991
Mansoura University Lecture 01/01/1983 01/01/1986
ETH- Zürich, Switzerland. Assistant II, Pharmazeutisches Institut 01/01/1979 01/01/1982
Mansoura University Assistant Lecture 01/01/1975 01/01/1983
Assuite University Assistant Lecture 01/01/1973 01/01/1974
Assuite University Teaching Assistant 01/01/1967 01/01/1973

Researches /Publications

Identification and isolation of anti-pneumonia bioactive compounds from Opuntia ficus-indica fruit waste peels. - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Bishr MM, Abdel-Aziz MM

01/05/2020

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Ammi Visnaga L., a Potential Medicinal Plant: A Review. - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Bishr M, Desouky S

01/01/2020

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• LC/MS of the soft coral Sarcophyton ehrenbergi and its isolated associated endophytic fungi: Aspergillus oryzae and Aspergillus flavus. - 01/1

OSAMA MOUSTAFA MOHAMED SALAMA

Elkhawas YA, Mostafa NM, Elissawy AM, Alnaggar MS, Kamal EM, Bishr MM, Singab AN

01/12/2019

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Effect of Salicylic acid foliar spraying on growth parameters, γ-pyrones, phenolic content and radical scavenging activity of drought stressed Ammi visnaga L. plant - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Moshera El Sherei, Dalia A. Al-Mahdy, Mokhtar Bishr

01/08/2019

The present study was conducted to evaluate the impact of drought stress and foliar spraying of salicylic acid (SA) on the secondary metabolites particularly the γ-pyrones and total polyphenolic content in the different organs of Ammi visnaga L. plant. The following were measured: different growth parameters, γ-pyrones, total polyphenolic content (TPC) and the antioxidant activity of the methanolic extracts. From the results obtained, it was clear that drought stress had a negative impact on growth of the plant and on the yield of the fruits, whereas it caused an increase in the percentage of the two major γ-pyrones: khellin and visnagin in most organs. The adverse effects of drought stress on growth parameters was found to be partially alleviated by the salicylic acid foliar spray. On the other hand, combination of SA foliar spray and normal irrigation gave the highest percentage of khellin (1.544 ± 0.002%) and visnagin (0.902 ± 0.002%), as well as an increase in the yield of fruits per plant. In contrast, drought alone and in combination with SA significantly (p < 0.001) increased the polyphenolic content and the radical scavenging activity. The highest polyphenolic content was recorded in the water stressed aerial parts sprayed with 2 mM SA, where it reached 78.28 ± 0.14 mg/gm dry weight calculated as gallic acid equivalent (GAE). Antioxidant activity, using DPPH assay, was measured for the different plant organs under different treatments where a reduction from 12.967 ± 0.983 to 2.803 ± 0.262 μg/ml in the IC50 was noted in the drought stressed aerial parts sprayed with 2 mM SA vs the normally irrigated plant. UPLC/MS analysis was used to demonstrate the effect of SA foliar application on the γ-pyrones and total polyphenolic content in Ammi visnaga L. fruits.

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Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare L. essential oil - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Sahar Fikry

01/03/2019

Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganisms. GC/MS of OV EO allowed the identification of 32 compounds representing 99.94% of the oil. The two major identified compounds were terpinen-4-ol (38.35%) and trans-sabinene hydrate (10.06%). Different methods were employed to illustrate the biostatic activity of OV EO. Results of the biostatic studies on OV EO using agar and broth dilution methods showed that Staphylococcus aureus (S. aureus) was the most sensitive organism; with a Minimum inhibitor concentration (MIC) 1.18 mg/ml. Agar diffusion method showed that the highest activity was observed against Bordetella bronchiseptica (Br. bronchiseptica), Saccharomyces cerevisiae (S. cerevisiae), Bacillus subtilis (B. subtilus) and Staphylococcus epidermidis (S. epidermidis) with inhibition zones 38 ± 1.5, 29.5 ± 0.8, 26.9 ± 0.9 and 26.9 ± 1.1 mm, respectively. Studying the dynamics of 1% v/v OV essential oil emulsion over a period of 6 h revealed that Escherichia coli (E. coli), B. subtilis, S. epidermidis and S. cerevisiae had the fastest response. Also increasing concentrations of OV oil emulsion increased the rate of cell killing and the duration of growth lag phase increased correspondingly. These data indicated that OV EO produces a concentration and time-dependent antimicrobial activity.

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Bioassay guided fractio-nation and cytotoxic activity of Daucus carota var. boissieri - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Mohamed Ashour, Abdel Naser Singab

01/06/2018

The hexane extract and the hydro-distilled essential oil from red carrot fruits (Daucus carota var. boissieri) were evaluated for their cytotoxic activity against human tumor breast cell lines (MCF-7). Cell viability was evaluated by MTT assay. The extract exhibited good cytotoxic activity shown through its low IC50 (9.12 ± 0.58 μg/ml) against the standard 5-Flououracil (8.46 ± 0.63 μg/ml). Phytochemical investigation of the hexane extract using column chromatography yielded three compounds; 8-methoxypsoralen (1), α-asarone (2) and 3,4,5-trimethoxy-benzaldehyde (3), a compound isolated for the first time from D. carota and from family Apiaceae. Structure elucidation of the isolated compounds was carried out on the basis of their spectral data analysis (IR, MS, 1H NMR an 13C NMR) The three isolated compounds were evaluated for their cytotoxic activity using the same conditions. Only compound (1) exhibited good cytotoxic activity (IC50; 9.38 ± 0.78 μg/ml), compound (2) had moderate activity (46.12 ± 1.31 μg/ml), while compound (3) had no cytotoxic activity (100.6 ± 3.11 μg/ml). These compounds need to be more investigated against other cell lines; also they are considered as a good substrate for future SAR study and modifications to produce more potent cytotoxic derivatives

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Supercritical fluid extraction of γ-Pyrones from Ammi visnaga L. fruits - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Mokhtar Bishr, Mohamed El-Degwy, Mohammed Abdel Hady, Mohamed Amin

01/06/2018

Extraction with supercritical fluid technique has proved to be effective in many applications including extraction and separation of various active principals from medicinal plants. It was used due to its advantages especially safety, specificity, selectivity and ease of component recovery. Ammi visnaga, L. belongs to the family Apiaceae. The fruits are used specifically for the treatment of kidney stones depending on its γ-Pyrones (mainly khellin and visnagin) [2]. The supercritical fluid extraction technique of khellin and visnagin was investigated and the operating conditions for their extraction were optimized. The effect of different pressure (150, 200, 300, 400 and 500 bars), temperature (35, 40, 45, 50 and 55 °C), and particle sizes of the raw material (0.5, 1, 1.4 mm and entire fruits) on the extract yield was studied under dynamic conditions for extraction for a run time of 90 min. Optimum supercritical extraction condition was found to be 200 bars at 45 °C and optimum particle size was found to be 1.4 mm. The yield is yellowish white bitter powder and measures 1.74% w/w relative to the dried weight of the fruits containing 38.414% w/w average γ-Pyrones content of which 29.4%w/w khellin, and 9.014%w/w visnagin.

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Chemical composition and antimicrobial activity of the essential oils of selected Apiaceous fruits - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Mohamed Ashour, Sahar Fikry, Abdel Naser Singab

01/06/2018

Antimicrobial properties of plants essential oils are continuously investigated to use them as potential drug candidates to overcome the problem of microbial drug resistance. The aim of this research is to study the antimicrobial effects of the essential oils of ten Apiaceous fruits [Pimpinella anisum L. (anise), Carum carvi L. (caraway), Apium graveolens L. (celery), Coriandrum sativum L. (coriander), Cuminum cyminum L. (cumin), Anethum graveolens L. (dill), Foeniculum vulgare L. (fennel), Petroselinum crispum L. (pasley), Daucus carota L. var. sativus (yellow carrot) and Daucus carota L. var. boissieri (red carrot)]. Results of agar-well diffusion method revealed that the maximum inhibition zones were obtained with cumin, coriander and caraway oils against the standard bacterial strains Escherichia coli, Bordetella bronchiseptica followed by Staphylococcus aureus. Results of viable count time-kill method revealed that coriander oil had the highest antimicrobial activity with more than 99.99% killing of the exposed cells of the standard E. coli and Bordetella bronchiseptica standard strains. GC/MS was carried out to identify the chemical composition of the most active oils. The percentage of identified compounds by GC/MS was 92.5%, 99.43% and 98.66% for cumin, coriander and caraway oils, respectively. Monoterpenes were the most abundant components in the three oils.

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Foliar spraying of salicylic acid induced accumulation of phenolics, increased radical scavenging activity and modified the composition of the essential oil of water stressed Thymus vulgaris L - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Mostafa Fekry, Mokhtar Bishr, Soheir El-Zalabani

01/02/2018

Polyphenolic compounds are considered valuable secondary plant metabolites owing to the myriad of biological activities they exert. This study aimed to investigate the effect of applying various concentrations of the plant growth regulator, salicylic acid (SA), on Thymus vulgaris L. while subjecting the plant to decreasing amounts of irrigation water. The following parameters were monitored; total polyphenolic and flavonoid content, yield and composition of the essential oil, and antioxidant activity of the alcoholic extracts. Drought alone significantly (P < 0.05) increased the polyphenolic and flavonoid content, yield of the essential oil and antioxidant activity. The total flavonoid content in control plants was 6.1 ± 0.3 mg/gm dry weight calculated in terms of rutin equivalent. However, in drought stressed plants, (irrigated at 25% of the field capacity) sprayed with 3 mM SA, the flavonoid content increased to 32.1 ± 0.1 mg/gm dry weight calculated in terms of rutin equivalent. Moreover, the total phenolic content increased from 8.5 ± 0.3 to 68.5 ± 1.2 mg/gm dry weight calculated in terms of gallic acid in the same test plants. Radical scavenging activity, using DPPH assay, was measured for the different plant treatments. A decrease from 74.4 ± 0.4 μg/ml to 36.6 ± 0.9 μg/ml of IC50 was recorded in the drought stressed plants (25% FC) sprayed with 3 mM SA compared with the control plants. The variability in polyphenolic composition between the control plants and plants with the highest total polyphenolic content was investigated by UPLC-ESI-MS/MS. Rosmarinic acid was detected as the major component in samples from both treatments, with a higher percentage observed upon subjecting the plant to the test conditions (25% FC and sprayed with 3 mM SA). The highest yield of the essential oil (1 ± 0.06 %v/w) was obtained from drought stressed plants (25% FC) sprayed with 2 mM SA. GC/MS analysis of oil samples revealed that the Thymol content increased with drought stress, while that of p-cymene decreased. However, an increase of p-cymene was witnessed as a result of SA spraying.

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LC-ESI-MS/MS and cytotoxic activity of three Pistacia species - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Elkhawas. Y A, Kirollos. F N

01/01/2018

LC-ESI-MS/MS was used for a comprehensive characterisation of ethanol extract from the leaves of three Pistacia species. After optimisation of the method and the use of the negative ionisation mode, a total of 42 different compounds were identified, of which 22 were tentatively characterised in P. chinensis Bunge, 33 in P. khinjuk stocks and 25 in P. lentiscus L. leaves. Flavonoids, phenolic acids, and their derivatives were the most abundant identified compounds. LC-ESI-MS/MS revealed identification of 15, 18 and 6 not previously detected compounds in P. chinensis Bunge, P. khinjuk Stocks and P. lentiscus L., respectively. The three extracts were also tested for their cytotoxic activities against human PC3 prostate cancer, A549 lung cancer, MCF7 breast cancer and HepG2 liver cancer. Generally, all the extracts have a moderate cytotoxic activity against lung, breast and prostate cancer, with different IC50. However, only P. lentiscus L. showed moderate activity against liver cancer.

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Chemical composition and antimicrobial activity of essential oils of selected Apiaceous plants growing in Egypt - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

S Fikry, A NaserSingab

01/05/2016

Antimicrobial properties of essential oils of plants are continuously being investigated in order to use them as potential drug candidates to overcome the problem of microbial drug resistance [1]. The aim of this research is to study the antibacterial and antifungal effects of ten traditional Apiaceous essential oils; Pimpinella anisum L. (Anise), Carum carvi L. (Caraway), Apium graveolens L. (Celery), Coriandrum sativum L. (Coriander), Cuminum cyminum L. (Cumin), Anethum graveolens L. (Dill), Foeniculum vulgare (Fennel), Petroselinum crispum L. (Parsely), Daucus carota L. var. sativus (Red Carrot) and D. carota L. var. boissieri (Yellow Carrot).GC/MS was carried out to identify the chemical composition of the most active antimicrobial essential oils. Results of agar-well diffusion method revealed that the maximum inhibition zones were obtained with cumin, coriander and caraway oils against the standard bacterial strains Escherichia coli, Bordetella bronchiseptica followed by Staphylococcus aureus. On the other hand, the remaining essential oils showed much smaller inhibition zones or no inhibition at all. The three most active essential oils; cumin, caraway and coriander were selected to confirm their antibacterial activity by using viable count time-kill method. Results of this experiment revealed that the coriander essential oil had the highest antimicrobial activity with more than 99.999% killing of the exposed cells of the standard E. coli and Bordetella bronchiseptica standard strains during 5 minutes exposure. These results are in accordance with previously published data [2]. The percentage of identified compounds by GC/MS was 92.5, 99.43 and 98.66 for the cumin, coriander and caraway oils respectively. Monoterpenes were the most abundant components in the three oils.

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Phytochemical and biological evaluation of Cassia tora, L. seeds, IOSR-Journal of Pharmacy and Biological Sciences. - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Fathalla N.; Bishr M.; Singab Abdel N.

01/01/2015

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Phytochemical and biological evaluation of Cichorium intybus L. seeds - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Fathalla N.; Bishr M.; Singab Abdel N.

01/01/2015

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Chemical Composition and biological activity of the essential oils obtained from yellow and red carrot fruits cultivated in Egypt - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Khalil N.; Ashour M.; Singab Abdel N

01/01/2015

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Characterization of blue green algae isolated from Egyptian rice field with potential anti-hepatitis C active components - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Amer R. A.; Abdel Wahab A.; S. M. Fathy ; El Demellawy M.,

01/01/2013

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Characterization Of Fennel Fruits: Types And Quality (1) - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

01/01/2012

Four samples of different fennel fruit cultivars (F1- F4), obtained from El Fayoum, Egypt (F1), El Menia, Egypt (F2), Sudan, El Khartoum (F3) and Germany (F4) were cultivated in MEPACO's farm (Arab co. for Pharm. And Med. Plants, Cairo, Egypt) and the obtained fruits were subjected to macro and micro morphological stereo-microscopic examination as well as GC-MS analysis of their volatile oils. The aim of the study is to determine the differences in the macro and micro characters of different fruit cultivars as well as their oil constitutes. The results show different exomorphic parameters Viz. shape, color, dimensions and surface sculpture. Also the stereo-microscopic examination showed differences in the epicarp, mesocarp, vitti and endosperm. GC-MS analysis of volatile oils of (F1 – F4) showed on comparing three parameters: fenchone, estragole and trans-anethole that F4 has the hihest percentage of trans-anethole (78.98%), while F1 and F2 have close values (1.05 and 1.02%, respectively) followed by F3 (3.02%). F4 has the lowest percentage of estragole (3.97%), while (F1-F3) have higher values (78.58, 64.81 and 25.79 %, respectively). Also F4 has doubled the percentage of fenchone (6.73%) of F1 and F2 (2.54 and 2.57%, respectively), while F3 has 0.69%. Thus results show that the two cultivars growing in Egypt (F1 and F2) have almost the same ratios of the compared parameters while the Sudan cultivar F3 is closer to F2 and F2 than it is to F4. Also the 3 cultivars (F1-F3) are far from specification of sweet fennel oil but close to bitter fennel oil. The German cultivar (F4) has the best oil quality as a sweet fennel. Investigation of the powdered samples (F1-F4) showed that only F4 is different in having higher abundant fragments of reticulate parenchyma cells with ratio of 2:3. In Conclusion: These findings are of pharmaceutical – industrial value helping in the production of herbal pharmaceutical products of fennel fruit and/or oil of known higher quality.

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Emulsion and rectal formulations containing myrrh essential oil for better patient complains - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

Etman M.; Amin M.; Nada A. H.; Shams-Eldin M.

01/01/2011

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Osama Salama, Ratan K. Chaudhuri and Otto Sticher, - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

01/01/1981

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O. Sticher and O. Salama, - 01/0

OSAMA MOUSTAFA MOHAMED SALAMA

01/01/1981

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