Dalia Samuel Shaker Kirolos

Shaker DS, Ishak RA, Elhuoni MA, Ghoneim AM.

01/03/2020

Dalia Samuel Shaker Kirolos

Dalia S. Shaker, Rania A. H. Ishak, Amira Ghoneim and Muaeid A Elhuoni.

01/07/2019

Dalia Samuel Shaker Kirolos

Heidi M. Abdel-Mageed, Shahinaze A. Fouad, Mahmoud H. Teaima

01/01/2019

This study was designed to optimize the effect of operating conditions and formulation parameters using various additives to develop a-amylase nanoparticles. a-Amylase was chosen due to its importance in the substantial number of industrial processing with emphasis on pharmaceutical industry. Factorial statistical design was adopted to effectively optimize the size, yield value, residual enzyme activity, and morphology of a-amylase nanoparticles using Nano Spray Dryer B€UCHI B-90. The physicochemical characterization of the prepared nanopowder was carried out using zetasizer and scanning electron microscopy (SEM) and enzyme activity assay. Results showed that the type of additive and mesh size significantly influenced the particles size and yield value. SEM images showed three different structure patterns where particle morphology was influenced by TweenVR 80 or sucrose at low concentration (0.05%). Optimized spherical nanoparticles (600nm) was obtained using 7 mm mesh cap size, sucrose (0.15%), 95% yield value, drying flow rate (100 L/min), and inlet temperature of 80
C. Higher storage stability was detected for enzyme spray-dried using larger cap size. It was concluded that nano spray drying of aqueous enzyme solution under determined operating conditions produced stable a-amylase powders. This would extend the application of the enzyme in a variety of pharmaceutical products.

Dalia Samuel Shaker Kirolos

DS Shaker, S Ismail, S Hamed, EM El-Shishtawy

01/12/2018

Terbinafine Hcl (TB) is a poorly water soluble antifungal drug. Topical nanoemulsion based gel containing TB was prepared with a view to improve its solubility and antifungal activity. In preparation of the nanoemulsion (NE), excipients were selected based on the solubility study. Peceol was optimized as the oil phase. Tween 80 and propanol were optimized as the surfactant and co-solvent respectively, and were mixed (Smix) in different weight ratios (1:1, 1:2, 1:3, 1:4, 4:1, 3:1 and 2:1, respectively). Pseudoternary phase diagrams were developed and Pecol and Smix were mixed in different weight ratios ranging from 1:9 to 9:1. Based on the NE region of each diagram, the formulae were selected. The formulated nanoemulsions were characterized and evaluated for in vitro drug release and thermodynamic stability. The optimum nanoemulsion formulae containing 10 or 15% w/w oil, 45% w/w Smix (1:2/1:3) and 45-40% w/ w aqueous phase) were incorporated into Carbopol 940 gel bases forming three different TB nanoemulsion based emulgel formulae (F1-F3) which were examined for ex vivo drug permeation and in vivo antifungal activity compared to the marketed product; Lamisil® emulgel. The results showed that TB skin permeation from all the prepared nanoemulsion based gel formulae was significantly (p < 0.05) improved in relation to the commercial emulgel. F3 exhibited a superior in vivo antifungal activity over the marketed emulgel for the treatment of Candida infection.

Dalia Samuel Shaker Kirolos

Maha E. Elmataeeshy , Magda S. Sokar , Mohamed Bahey El-Din , Dalia S. Shaker.

01/06/2018

Dalia Samuel Shaker Kirolos

Heidi Mohamed Abdel Mageed, Afaf S. Fahmy, Dalia S. Shaker, Saleh A. Mohamed

01/01/2018

One of the main challenges for successful pharmaceutical application of Catalase (CAT) is maintaining its stability. Physical immobilization of CAT through nano-encapsulation was proposed to resolve this challenge. CAT encapsulating niosomes (e-CAT) were prepared using Brij® 30, 52, 76, 92, and 97 in presence of cholesterol (Ch) by thin film hydration method. Niosomes were characterized for encapsulation efficiency % (EE), size, poly-dispersity index (PI) and morphology. Kinetic parameters, pH optimum, thermal stability and reusability of CAT were determined. The influence of optimized e-CAT dispersion onto thermally injured rat skin was evaluated. Results revealed that encapsulation enhanced CAT catalytic efficiency (Vmax/ Km). Free CAT and e-CAT had pH optimum at 7.0. e-CAT exhibited improved thermal stability where it retained 50% residual activity at 60 ◦C. Free CAT lost its activity after 3 consecutive operational cycles however, e-CAT retained 60% of its initial activity following 12 cycles. After 24 hr of topical application on thermal injury, a significant difference in lesion size was observed with e-CAT compared to control group. Based on these encouraging results, CAT immobilization demonstrated a promising novel delivery system that enhances its operational stability. In addition, nano-encapsulated CAT can be anticipated to be beneficial in skin oxidative injury.

Dalia Samuel Shaker Kirolos

Dalia S. Shaker, Mohamed A. Shaker, Mahmoud S. Hanafy.

01/07/2016

One of the main challenges for using Tamoxifen citrate (TMC) in breast cancer therapy is achieving proper target and efficient delivery of adequate concentration to the adenocarcinoma without harming healthy glandular and soft fatty tissue. Herein, TMC niosomal thermosensitive hydrogels were proposed as a tool to resolve this challenge. Niosomes were prepared by film hydration technique and incorporated into Pluronics thermosensitive gels prepared using cold method. The prepared hydrogels were evaluated for gelation temperature, rheological behavior and in vitro drug release. Moreover, in vivo anti-tumor activity was examined in Ehrlich carcinoma mice model through reporting solid tumor volume regression and tissue distribution of TMC. Type and ratio of used poloxamers were manipulated to provide the optimal gelation temperature (34e37
C). Rheological analysis showed low viscosity and elasticity values at low and room temperature while these values significantly increased at the physiological temperature. A prolonged diffusion-driven release of TMC was detected. In vivo data showed, evidently, that anticancer activity was improved with significant retention of the drug at the tumor site. These encouraging results confined that this in situ hydrogel depot offers an attractive approach for controlled delivery of TMC and clinically expected to be useful delivery system in loco-regional therapy for breast cancer.

Dalia Samuel Shaker Kirolos

Dalia S. Shaker, Mohamed A. Shaker, Mahmoud S. Hanafy.

01/07/2015

One of the main challenges in Tamoxifen cancer therapy is achieving localized, efficient and sustained delivery without harming normal healthy organs. This study focused on evaluating Tamoxifen Citrate (TMC) niosomes for localized cancer therapy through in-vitro breast cancer cytotoxicity as well as in-vivo solid anti-tumor efficacy. Different niosomal formulae were prepared by film hydration technique and characterized for entrapment efficiency % (E. E), vesicle size, morphology, and in-vitro release. The cellular uptake and anti-cancer activity were also tested in-vitro using MCF-7 breast cancer cell line. Moreover, in-vivo anti-tumor efficacy was examined in Ehrlich carcinoma mice model through reporting solid tumor volume regression and tissue TMC distribution. The obtained niosomes prepared with Span 60: cholesterol (1: 1 molar ratio) showed a distinct nano-spherical shape with EE up to 92.3% ± 2.3. Remarkably prolonged release of TMC following diffusion release behavior was detected. The optimized formula showed significantly enhanced cellular uptake (2.8 fold) and exhibited significantly greater cytotoxic activity with MCF-7 breast cancer cell line. In-vivo experiment showed enhanced tumor volume reduction of niosomal TMC when compared to free TMC. Based on these results, the prepared niosomes demonstrated to be promising as a nano-size delivery vehicle for localized and sustained TMC cancer therapy.

Dalia Samuel Shaker Kirolos

01/01/2013

ABSTRACT Objective: Transdermal patch is a promising approach that allows continuous input of drugs with short biological half-lives. The present study was designed to evaluate the short t1/2- lornoxicam (LX) transdermal patches through in-vitro skin permeation, skin irritation and biological evaluation on rat induced paw edema. Methods: LX patches were prepared using different polymer blends and plasticizers. The effect of Span80 and Transcutol® as permeation enhancers in absence and presence of oleic acid (OA), isopropyl myristate (IPM), triacetine and castor oil, on transdermal permeation through rat skin, was investigated. The safety of LX patches was evaluated through skin irritation study. The biological evaluation regarding the antiinflammatory effect of LX patches on rat paw edema was tested. Results: The following were the principal findings of this research. First, there was very good correlation between LX flux and the presence of IPM, Oleic as well as propylene glycol compared to other oils and triacetine. Second, span80 had significantly improved LX permeation from Eudragit blends (E100). while combining transcutol- castor oil showed no remarkable increase in drug flux. Third, the primary irritancy index (PII) proved the non-irritancy of the drug or any of the film components and showed that the innovated films are safe to be applied to skin for the intended period of time. Finally, LX patches had significantly inhibited the carrageenan induced rat paw edema compared to oral treatment. Conclusion: This study has supplied us with brightening results concerning the questionable equipotent therapeutic efficacy of transdermal versus oral LX and not irritant to skin. Keywords: Lornoxicam, Transdermal patches, Inhibition of edema, Irritation test

Dalia Samuel Shaker Kirolos

01/01/2013

Pharmacodynamic activity in transdermal patches

Dalia Samuel Shaker Kirolos

01/04/2008

The use of silicone as inert phase has no interaction with cyclodextrins was investigated to calculate the distribution coefficient